1Department of Biochemistry & Biochemical Engineering, JSBB, Sam Higginbottom University of Agriculture, Technology and Sciences (SHUATS), Allahabad, Uttar Pradesh, India
2Department of Biological Sciences, Sam Higginbottom University of Agriculture, Technology and Sciences (SHUATS), Allahabad, Uttar Pradesh, India
Online published on 5 July, 2018.
Protease enzymes have immense commercial value and play a pivotal role in application of various industrial sectors. Microbial proteases are one of the important groups of industrially and commercially produced enzymes which have several applications. In this study 148 bacterial strains were isolated from 50 different soil samples of slaughter house, fish market and sewage wastes of Lucknow, Uttar Pradesh, India. Out of which thirty eight strains competent of secreting extracellular alkaline protease. In preliminary screening the isolate SSB2 showed highest ability to hydrolyzed casein and skimmed milk which was done on skim milk agar media. Based on biochemical test the isolate showed positive for casein, starch, catalase and negative for gram staining, indole, methyl red, voges proskauer, gelatin, urea, oxidase, hemolysis and triple sugar iron test and found to be non motile. Strain SSB2 with the maximum yield alkaline protease was identified as Acinetobacter variabilis based on nucleotide homology and phylogenetic analysis (16S rDNA sequencing). Protease production was enhanced by optimizing the culture conditions. Many physical parameters were studied to optimize the maximum yield of alkaline protease. The maximum enzyme activity were observed with optimum incubation time, temperature, pH, carbon, nitrogen sources, NaCl and metallic ions were determined as 36 h, 37°C, pH 11.0, 1% glucose, 1% yeast extract, 1M NaCl, and 1mM Zn2+, respectively for protease production. The study revealed that the strain of A. variabilis is a potent source of alkaline protease. In consequence, such additions can supplement alkaline protease production and their application in various industries.
A new potent alkaline protease producing strain Acinetobacter variabilis was isolated from the soil sample of slaughter house, Lucknow region, Uttar Pradesh, India
The study concluded that A. variabilis has a wide scope for the industrial production of alkaline protease. Further studies needed to ascertain the potential applications
Alkaline protease enzyme, 16S rDNA, Acinetobacter variabilis, soil samples
