1Animal Biotechnology Centre, Nanaji Deshmukh Veterinary Science University, Jabalpur, Madhya Pradesh, India
2Chairman, M.P. Private Universities Regulatory Commission, Bhopal, Madhya Pradesh, India
*Corresponding author: drhemlatajain@gmail.com (ORCID ID: 0000-0002-9707-1440)
Online published on 22 March, 2019.
Transfected cell lines are widely used in biological research. Cell heterogeneity within a population can be vital to its unusual function and fate. Mixed mutants may not be as informative regarding which cell responds to our treatment. Cell to cell differences in RNA transcripts and protein expression further complicate the condition. To understand the real affect on the treated cells, researchers want to have single cell monoclonal population to provide correct information for getting truly transfected cell population. With this fact, we focus on the single cell isolation from transfected cells and its proliferation, which include methods of single cell pickup, analysis of media supplements on growth parameters and optimizing the best condition for single cell proliferation. Dilution method and Single cell pickup with mouth pipette method were compared for their efficacy. Rotational media supplement was found better than the regular medium used for cell growth. To explore the best suitable conditions for cell proliferation and survival, the cell viability percentage, total cell count, cell proliferation rate and population doubling time were calculated at different time interval in different media supplement groups.
Single cell pickup is crucial for RNA interference studies to get monoclonal population
Variable media supplementations perform better than the routine media.
Single cell proliferation, Clonal cell population, Dilution method, Mouth pipette cell pickup, Rotational media
