1Department of Fruit Science, Dr. YS Parmar UHF, Nauni, Solan, Himachal Pradesh, India
2Management Expert-Horticulture, HP-SHIVA, Directorate of Horticulture, Navbahar, Shimla, Himachal Pradesh, India
3National Dairy Research Institute, Karnal, Dairy Economics Division, Haryana, India
*Corresponding author: soniasharma75059@gmail.com (ORCID ID: 0000-0002-8222-6294)
Online published on 4 March, 2020.
Genetic similarity of fifteen strawberry cultivars was assessed using RAPD and ISSR molecular markers. The DNA of the fifteen strawberry cultivars were extracted and amplified by PCR with RAPD and ISSR primers. The DNA Fragments were separated in agarose gel for RAPD and in polyacrylamide gel for ISSR markers. The genetic similarity matrix was estimated by Jaccard coefficient. Studied cultivars were found to be coherent into two groups. RAPD markers projected more coherence with origin and genealogy of the cultivars. ISSR markers projected more similarity between ‘Chandler’ and ‘Shasta’ whereas as per RAPD marker ‘Chandler’ and ‘Sweet Charlie’ were more closely related. In molecular assessment Elyana cultivar was found to be marked cultivars as it shows long flowering duration, higher yield and bigger fruits and can be used as a potential genotype to breed cultivars among the locally adapted cultivars.
The present study involves the Molecular Characterization using RAPD and ISSR markers to assess the genetic similarity of fifteen strawberry cultivars.
‘Elyana’ cultivar was found to be distinct from other cultivars based on molecular assessment because of its long flowering duration, higher yield and bigger fruits therefore, may be considered as a potential genotype.
Fragaria × ananassa Duch, Genetic Variability, Jaccard's Coefficient, Dendrogram, Informative Primers, Polymorphic Bands
