1Plant Biotechnology and Molecular Biology Lab, Department of Biosciences, Saurashtra University, Rajkot, Gujarat, India
2Vimal Research Society for Agro-Biotech & Cosmic Powers, 80 feet road, Aji Area, Rajkot, Gujarat, India
*Corresponding author: thakervs@gmail.com (ORCID ID: 0000-0001-8596-3604)
Online Published on 25 July, 2023.
The existence of metabolites that interfere with isolation procedures and downstream applications makes plant RNA extraction difficult. The current research used a standardized RNA extraction protocol from maize (Zea mays L.). We developed a protocol for extracting pure RNA from plant tissues using both extraction buffer and the TRIzol reagent, and show that this RNA extraction method works not only at Low temperatures but also at room temperatures, making it the easiest and most efficient method for extracting pure and undegraded RNA directly from tropical plants in the field. RNA isolation methods based on our modified protocol yielded good results in maize leaf, seed, flowers and other grass species. The isolated RNA was found to be suitable for both PCR and RT-PCR amplification and transcriptome analysis. The method is repeatable and can be used to isolate high-quality RNA and conduct gene expression studies. RNA extraction paves the way for deciphering the complex regulatory network involved in multiple stress responses by studying gene-environment interactions at the transcriptome level.
• This study provides simple and rapid protocol for RNA extraction from maize and Poaceae grasses.
• The isolated RNA shows good quality and concentration.
• BioAnalyzer quantification provided good RIN value, which shows utility for various downstream applications like transcriptome analysis.
RNA extraction, Poaceae plants, TRIzol reagent, Downstream applications
