1Division Microbial and Environmental Biotechnology, Aspee Shakilam Biotechnology Institute, Navsari Agricultural University, Surat, Gujarat, India
2Department of Biosciences, Phytochemical, Pharmacological and Microbiological Laboratory, Saurashtra University, Rajkot, Gujarat, India
*Corresponding author: hpatel@nau.in (ORCID ID: 0000-0002-5075-1115)
Online Published on 27 July, 2023.
The aim of this study was to determine minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of active fraction isolated from methanol extract of Polyalthia longifolia against 70 clinically isolated Staphylococcus strains. Two different fractions (Fraction 1 and fraction 2) were isolated from methanol extract of P. longifolia and studied for anti-staphylococcal activity by agar well diffusion method. Fraction 2 showed considerable anti-staphylococcal activity; hence it was selected for MIC and MBC studies by 96 well microtitre plates. Rifampicin was used as positive control. Fraction 2 was highly active against most of the strains studied which was comparable with standard drug rifampicin. Our results confer the utility of this plant fraction in developing a novel broad spectrum anti-Staphylococcus agent.
• In the present investigation, two fractions of methanolic extract of Polyalthia longifolia was used to check its antibacterial potential against 70 clinically isolated Staphylococcus strains.
• Anti-staphylococcal efficacy of fraction 2 was more potent than fraction 1 against most of the strains.
Polyalthia longifolia, Anti-staphylococcal activity, MIC, MBC, Plant extract
