1 Plant Tissue Culture and Molecular Laboratory, Dept. of Genetics & Plant Breeding, University of Agricultural Science, Raichur, India
2Dept. of Horticulture, University of Agricultural Science, Dharwad, India
3Dept. of ABM, University of Agricultural Science, Dharwad, India
4Dept. of Agricultural Engineering, IIT, Kharagpur, India
5Dept. of Biotechnology, GKVK, University of Agricultural Science, Bangalore, India
6Dept. of Crop Physiology, University of Agricultural Science, Dharwad, India
*Email: shashidhara.gpb@gmail.com
Tissue culture work in sesame is scanty and started late. As on date only few reports are available in the line of tissue culture of sesame. In terms use of good and costly growth regulators particularly Thidiazuran is not tried till today, even those, their results are shown better in other crops but till today there is no reports are available in this line. Thidiazuran (TDZ), a cytokinin-a synthetic phenylurea, is considered to be one of the most active cytokinins for shoot induction in plant tissue culture and known to induce shoot regeneration from different explants of many recalcitrant species. Several reports suggested that TDZ results in shoot regeneration better than other cytokinins. TDZ induced morphogenesis probably depends on the levels of endogenous growth regulators and TDZ modulates endogenous auxin levels. Multiple high frequency shoot induction both from meristamatic tissue and callus in several crops by TDZ is known.
Thidiazuran, Shoot regeneration, In vitro cell line selection, Sesamum indicum, Regeneration
