International Journal of Agriculture, Environment and Biotechnology

  • Year: 2012
  • Volume: 5
  • Issue: 3

Gas chromatographic-mass spectrometric (GC-MS) analysis of marigold (Tagetes erecta L.) flower oil

  • Author:
  • Deb Prasad Ray1,, S Srivastava2, R.P. Singh3
  • Total Page Count: 4
  • DOI:
  • Page Number: 215 to 218

1National Institute of Research on Jute and Allied Fibre Technology, Indian Council of Agricultural Research, 12, Regent Park, Kolkata, India

2Indian Institute of Natural Resins and Gums, Namkum, Ranchi, India

3Division of Agricultural Chemicals, Indian Agricultural Research Institute, New Delhi, India

Abstract

The essential oil of the marigold (Tagetes erecta L) flower petals was obtained from the flower collected from the experimental field of Indian Agricultural Research Institute, New Delhi. Theses flower petals were hydro distilled in a Clevenger apparatus to obtain the yellow coloured essential oil. The oil yield was 0.1% (w/w). This oil was collected and dried in a desiccator over calcium chloride. The dried oil was injected to a GC-MS. There were ten peaks revealed in the GC. The mass spectra of five out of ten compounds could be analyzed. The spectral fragmentation pattern revealed that the volatile constituents of marigold oil are mixture heterogeneous components. The compounds identified from the spectral analysis were as a-sesquiphellandrene (Rt 2.165 M+ 204), b-sesquiphellandrene (Rt 2.561, M+ 204), and 2-methyl-6-(4-methyl cyclo hexadienyl) hept -4-en-2-ol (Rt 3.701, M+ 216), myristoleic acid (Rt 3.986 M+ 226) and trieicosane (Rt 7.882, M+ 324). Structure of few other compounds could not be confirmed from the mass spectra.

Keywords

Marigold, Tagetes erecta, Essential oil, α-Sesquiphellandrene, β-Sesquiphellandrene