Characterization of Indian races of Fusarium oxysporum f.sp. ciceri through RAPD markers

In the present investigation four races (5 isolates) of Fusarium oxysporum f.sp.ciceri were screened by using 30 RAPD primers, off which 23 primers produced scorable bands. A total of 155 amplicons amplified with an average of 7 bands per primer. Off 155 bands, 149 bands were polymorphic and level of polymorphism was 96.12%. The primer OPA- 2 amplified maximum of 13 bands while primer OPA- 12 and primer OPB- 14 amplified minimum of 3 bands. The Race-1 was found to have higher value of similarity coefficient (0.6948) whereas Race- 3 was found to have lower value of similarity coefficient (0.3701). The analysis showed that Race- 3 is distinct from other races of Fusarium oxysporum f.sp. ciceri. The RAPD primers OPA- 13, OPA- 16, OPA- 18, OPB- 14 and OPB- 15 showed monomorphic banding pattern could be used for the development of the SCAR (Sequence Characterized Amplified Region) markers for identification of Fusarium oxysporum f.sp. ciceri