Molecular characterization in mustard [Brassica juncea L. Czern & Coss.]

Research was aimed to molecular characterization of parents and specific crosses through RAPD markers thus RAPD assessment on genetic variation of total 34 accession comprised six parental genotypes and their 28 F₁ hybrids of Indian mustard were analysed. The genomic DNA extracted from each genotype was used to PCR amplification with initially twelve primers were tested. Out of twelve seven primers viz., OPA-07, OPA-09, OPL-07, OPB-03, OPB-04, OPC-05 and OPC-09 yielded comparatively maximum number of amplification product with high intensity and minimal smearing, good resolution and also clear band. A total of 57 DNA fragments, out of which 48 were polymorphic given on average of 84.63 percent of polymorphism. The average polymorphism per band was 6.86 and per cent polymorphism varied between 71.43 (OPB-03) to 100 (OPL-07) percent. The seven primers and their total amplicons of 34 accession grouped into two clusters i.e cluster A, implicated all parental genotypes and cluster B, implicated All F₁ hybrids, cluster B over separated to three sub cluster i.e. B₁, B₂ and B₃. The genetic resemblance ranged from 0.32 to 0.96 which illustrated that significant genetic variation exists among various combinations of mustard lines.