1Department of Microbiology, VBS Purvanchal University, Jaunpur, India
2National Beareu of Agriculturally Important Microorganisms, Mau Nath Bhanjan, India
3School of Biological Sciences, Seoul National Uniersity, Seoul, Republic of Korea
4Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, India
5Department of Biotechnology, VBS Purvanchal University, Jaunpur, India
*Email: sptiwarimicro@gmail.com
Online published on 28 June, 2013.
Plant growth promoting rhizobacteria (PGPR) are known to influence plant growth by various direct or indirect mechanisms. Jaunpuri Giant Newar variety of radish was selected to study the PGPR profiling due to its peculiar growth features.The analysis of PGPR was based on their plant growth promotion (PGP) attributes, requirement of carbon substrates, amplified ribosomal DNA restriction analysis (ARDRA) and 16S rDNA homology. In search of efficient PGPR strains associated with radish cv. Jaunpuri Giant Newar rhizosphere, a total 115 isolates were randomly selected and screened for PGP attributes, out of which twenty isolates showing multifarious PGP traits were selected for detailed study. Metabolic characterization of representative strains revealed a large versatility with respect to carbon utilization. ARDRA revealed ten clusters composed of 1–5 strains. Based on 16S rRNA gene sequencing, the isolates were identified as Micrococcus luteus, Enterobacter cloacae, Bacillus subtilis, Pseudomonas sp., Agrobacterium tumefaciens, Leifsonia sp., P. aeruginosa, B. cereus, B. licheniformis, Nesterenkonia terrae. To the best of our knowledge, this is the first report from India as far as systematic, genetic and functional diversity of PGPR in radish rhizosphere region is concerned. Additionally, the generated information may serve as a baseline data for developing effective bio-inoculants to improve plant growth and biotic stress management of radish in an eco-compatible manner.
A total of 115 isolates were obtained from rhizospheric region of Radish cv. Jaunpuri Giant Newar.
Among115, 20 isolates showing multiple PGP traits were characterized by BIOLOG, RFLP and ARDRA.
Representative isolates from each group resulted from ARDRAwere identified by 16S rDNA sequence homology.
Most of the identified isolates were related to genera Bacillus followed by Pseudomonas, Enterobacter, Agrobacterium, Micrococcus, Leifsonia and Nesterenkonia.
ARDRA, biolog, PGPR, raddish, rhizobacteria
