Jatropha curcas, is an upcoming energy source, which promises to mitigate energy crisis and environmental pollution. Jatropha seeds (0.4–12 tons/ha/yr) contain oil (30–40%) which is non edible due to the toxins such as phorbol esters, trypsin inhibitors, lectins and phylates. Various combinations of auxins with cytokinins were used for regeneration study. The best shoot regeneration (80%) was observed in MS medium supplemented with NAA (0.125ppm) and BAP (1.5ppm). Root induction was successfully obtained in plane MS/MS with auxins. Acclimatization and hardening was quite successful with survival rate of 60%.
Biotype of plant and various plant parts shows variation in regeneration during tissue culture. Best shoot regeneration (80%) was observed in MS medium supplemented with NAA (0.125ppm) and BAP (1.5ppm) Root induction (90%) was successfully obtained in plane MS with survival rate of 70%
Biodiesel, jatropha curcas, micropropagation, regeneration
