Isolation and Assessment of Plant Growth Promoting Activity of Siderophore Producing Pseudomonas fluorescens in Crops

The Pseudomonas fluorescens a major Rhizobacteria encourage the plant growth through producing yellowish green fluroscent siderophore involve in high affinity transport of iron into the cell. The P. fluorescens isolates grow in iron deficient media because of production capacity of siderophore depends on iron content. Fifty nine Pseudomonas fluorescens were isolated in King's B media under 260 nm wavelengths from the rhizosphere and non-rhizospheric soil of cave, forest, fellow land and agriculture field in Chhattisgarh region. The amounts of siderophore produce by P. fluorescens isolates were screen in iron deficient succinate media and most of them were found positive for the production of much siderophores. One of the isolate from Pakhanjore area “P3” produce highest siderophore, which further assessed for plant growth promotion activities of crops rice, urd, mung and arhar. The study showed significantly higher increase in root length over control plants was observed in Rice (68.45%) followed by Urd (40.1%), Mung (33.71%) and Arhar (29.53%). Similarly, significantly higher increase in shoot length over control plants was observed in Rice (68.925%) followed by Urd (43.42%), Mung (39.96%) and Arhar (19.45%). It has been shown that these bacteria competively colonize plant roots and cause the plant statistically significant root and shoot increases by stimulating plant growth and reduce the incidence of plant disease of above mention crops under green house of tray culture conditions.

There were 59 isolates of pseudomonas fluorescens isolated from rhizosphere and non-rhizospheric soil.

P. fluorescens isolates were screen in iron deficient succinate media for amount of siderophore production.

One of isolate highest siderophore producing further assessed for PGPA.

The study showed significantly higher increase in shoot length and root length by P. fluorescens