1Department of Veterinary Public Health, College of Veterinary and Animal Sciences, Mannuthy, Kerala, India
2CMS College of Science and Commerce, Coimbatore, Tamil Nadu, India
3Department of Pelagic Fisheries, Central Marine Fisheries Research Institue, Ernakulam, Kerala, India
*Email: anu_C_jose@yahoo.com
Online published on 24 June, 2014.
Mangroves occupy a relatively unexplored site in endophytic microorganism isolation so they can represent a new source in obtaining more enzymes with different potentialities. The present study was carried out to evaluate the ability of Bacillus sp isolated from mangrove species Rhizophora mucronata for the production of Cellulase enzyme. After production, Proteins from the culture filtrate were precipitated by Ammonium Sulfate (60%). The precipitate was reconstituted with assay buffer and dialyzed against same buffer. Then the dialyzed sample applied on DEAE Cellulose column and eluted with 1M NaCl in buffer. Purified enzyme was found to be 65KDa by SDS PAGE. The optimum conditions for the enzyme activity were found to be at a temperature 37°C and PH 7.0. The Km and Vmax values of the enzyme were 1.25mg/ml and 14.6 IU/ml, respectively.
Mangrove endophytic bacteria was isolated and screened for cellulase activity.
Endophytic Bacillus sp. Isolated from Rhizophora mucronata used for the production of Cellulase enzyme.
Cellulase enzyme was purified by Ammonium Sulfate precipitation, Dialysis and Column chromatography.
The optimum conditions for the enzyme activity were found to be at a temperature 37°C and PH 7.0.
The Km and Vmax values of the enzyme were 1.25mg/ml and 14.6 IU/ml, respectively.
Endophytic bacteria, Rhizophora mucronata, cellulase enzyme
