1Department of Fruit Science, K R C College of Horticulture, Arabhavi, Karnataka, India
2Dept of Fruit Science, College of Horticulture, Bidar, Karnataka, India
3CRIDA, Santoshnagar, Saidabad PO., Hyderabad-500 059, Andhra Pradesh, India
4Crop Improvement and Biotechnology, College of Horticulture, Bagalkot, Karnataka, India
5Department of Biotechnology, ANGRAU, Hyderabad, Andhra Pradesh, India
*Corresponding author: manjupenki25@gmail.com
Online published on 29 September, 2014.
To compare the effect of 6-benzylaminopurine (BAP) and thidiazuron (TDZ) on morphological abnormal plants or per cent variant plantlets during secondary hardening period. Shoot tips of Musa spp. were cultured on MS medium supplemented with different concentrations (2.0 and 5.0 mg/L) of BAP and (0.2 and 0.3 mg/L) of TDZ and NAA 0.2 mg/L. Wherever, TDZ is present in the medium some morphological dwarf plants were observed. The maximum plant height (16.17 cm) and number of leaves (4.20) was in T2. Where, the maximum shoot diameter (4.97 mm), number of primary roots (4.53) and length of longest root (11.17 cm) was in T1. TDZ at 0.2 mg/L it increases the morphological abnormal plants like dwarf plants (20.00%). In conclusion BAP at 5.0 mg/L, TDZ at 0.2 mg/L and NAA at 0.2 mg/L and BAP at 2.0 mg/L, TDZ at 0.2 mg/L and NAA at 0.2 mg/L were assumed to be the most suitable for commercial micropropagation system with low frequency of abnormal shoot production for local banana cultivars.
BAP is the best for healthy, thinner and lengthy shoots.
Dwarfness is the major abnormality during hardening where ever TDZ is used.
Rooting (90–95%) was successfully obtained in NAA medium and survival of plantlets (70–75%) during hardening.
Morphological abnormal plants, cytokinins, micropropagation, Musa spp., shoot tip
