Biochemical Characterization and Molecular Fingerprinting of Plant Growth Promoting Rhizobacteria

Plant growth promoting rhizobacteria (PGPR) are a heterogeneous group of bacteria that can be found in the rhizosphere, which can improve the extent or quality of plant growth directly or indirectly. However, screening strategies for selecting the best rhizobacterial strain for rhizosphere competence with other microbial species in the plant rhizosphere will require more comprehensive knowledge. In present investigation nine different strains were tested for their PGPR properties by using RFLP analysis on 16S rRNA gene or amplified rDNA restriction analysis (ARDRA). 16 S rDNA amplification was done and restriction profiling was done using two endonuclease i.e. msp1 and taq1. Depending upon banding pattern of all the nine strains dendogram was created using NTsys software. A clear cut difference was seen in genetic diversity among the strains. Pseudomonas was found to be the most effective strain among all. Depending upon the outcome we can conclude that ARDRA can be effective tool for analyzing the genetic diversity among different bacteria and PGPR starin e.g. Pseudomonas, Bacillus can be used as a potent biofertilizer.

Nine Bacterial strains were obtained from Rhizospheric lab and were molecular characterized and sequenced.

ARDRA analysis of the diversity of strains revealed that strains are contained in two clusters,

Isolate P3 (Pseudomonas fluorescence) was found to be most efficient PGPR which solubilized insoluble phosphorus, produced IAA, siderophore, amylase, lipase and catalase.

A dendogram was constructed based on the restriction profile of nine strain restricted with Msp I and Taq I, revealed 14B, 8B, 9F and 13B had 100% similarity while strain 1B was placed distinctly in the tree (exhibiting less than 65% similarity).