Department of Microbiology and Biofertilizer Project, B. A. College of Agriculture, Anand Agriculture University, Anand, Gujarat, India
*Corresponding author: rachanaprogen@gmail.com
Online published on 9 January, 2015.
In the present investigation, plant growth promoting activities of mycopesticides (beneficial mycopesticides or biological control fungi) viz. Trichoderma, Paecilomyces, Beauveria and Metarhizium were assessed. Their efficacy for the growth enhancement of Groundnut was investigated through laboratory as well as by pot studies. Results of In vitro studies have shown their higher potential of P-solubilization these fungi. In the experiment, two native cultures and five fungal isolates (procured from MTCC) were used. P solubilization ability of the test mycopesticides have shown good solubilization zones by the PlA isolate on PKVK agar. Not only this, TvA and ThM has also shown good P solubilizing zone on PKVK medium - supplemented with rose bengal dye. In liquid culture medium, ThM showed the highest P solubilizing ability (309.33 μg/ml). HPLC analysis was carried out to determine the presence of organic acids in the culture supernatant of promising cultures. TvM showed presence of pyruvic acid, formic acid, orotic acid, citric acid and butyric acid. Efficacy of mycopesticides in pots study as PGP has revealed seed treatment as the best one, but recommended fertilizer was found significantly superior than all mycopesticides treatments. Amongst mycopesticides, Trichoderma spp. proved to be the best followed by Paecilomyces. Beauveria in most cases remained at par with Paecilomyces but was inferior to Trichoderma. Metarhizium was better than control but was inferior to other fungal treatments. Efforts clearly indicated that mycopesticides can significantly enhance groundnut growth and production as a plant growth promoting fungi (PGPF) proving their dual ability.
Trichoderma viride (Pers) (AAU1, TvA) and Paecilomyces lilacinus (Thom) Samson (AAU2, PlA) were used for the study.
In vitro study of phosphate solubilization activity of mycopesticides were determined
Efficacy of mycopesticides for growth enhancement of groundnut in pots were done
Mycopesticides, P solubilization, plant growth promotion, HPLC, groundnut