Faculty of Agriculture, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, West Bengal-741252, India
*Corresponding author: rup.biotech@gmail.com
Online published on 9 January, 2015.
Lathyrus sativus L. (Grasspea) is a protein rich pulse crop. Enough information about genetic diversity of Lathyrus germplasm is needed to improve this crop. Molecular characterization is one of the tools to identify the hidden genetic diversity. The present study was undertaken to reveal the genetic diversity in a set of 20 grasspea genotypes using RAPD (Randomly Amplified Polymorphic DNA) marker system. For the 15 primers, various efficiency parameters that include Polymorphism Information Content (PIC), Marker Index (MI), Resolving Power (RP) and Diversity Index (DI) were studied. Finally Principal coordinate analysis (PCoA) and dendrogram was perfomed to find out the genetic diversity among the germplasm. The screened RAPD markers will facilitate further studies in population genetics and utilization of this crop.
Selected RAPD markers are highly informative for these grasspea genotypes.
Four efficiency parameters of RAPD marker system were studied.
Three clusters were obtained on the basis of RAPD profiling.
Genetic diversity, grasspea, randomly amplified polymorphic DNA
