Department of Plant Biotechnology, College of Agriculture, Vellayani, Thiruvananthapuram, Kerala-695 522, India
*Corresponding author: alexswapna@hotmail.com
Online published on 9 January, 2015.
Banana Bunchy Top virus (BBTV) is a virus seriously affecting banana cultivation. Primers were designed for the amplification of coat protein gene for the diagnosis of BBTV in the commonly grown banana cultivars of Kerala, India, viz., Red Banana (AAA), Palayankodan (AAB), Dwarf Cavendish (AAA), Motta Poovan (AAB) and Ney Poovan (AB). PCR detection using these primers at an early stage can aid in disease free planting material production.
Primers designed for the early detection of Banana bunchy top viral infection using PCR produced an amplicon of size 500 bp in all the infected samples of the five varieties of banana.
PCR primers, BBTV detection, banana
