International Journal of Agriculture, Environment and Biotechnology
  • Year: 2015
  • Volume: 8
  • Issue: 2

Biochemical Investigation on Antioxidative and Antinutritional Characters of Yellow Seeded Brassica Genotypes for Quality Assessment

1Department of Biochemistry, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology, Pantnagar-263 145, U. S. Nagar, Uttarakhand, India

2Department of Molecular Biology and Biotechnology, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology, Pantnagar -263 145, U.S. Nagar, Uttarakhand, India

*Corresponding author: punetha_hp@rediffmail.com

Online published on 6 August, 2015.

Abstract

Yellow seeded Brassica is one of the most important oilseed crop cultivated in many parts of the world. The oil is consumed predominantly as edible oil and the defatted meal cake is utilized as animal/poultry feed. In the present study, intact seeds and defatted meal of 12 yellow-seeded Brassica genotypes evaluated for the presence of anti-oxidative and anti-nutritive factors. The maximum phenolic, orthodihydroxy phenols, flavonoid contents observed for Bio 39(16.946 mg/g), Bio 30(2.38 mg/g) and Bio 39(8.282 mg/g) repectively. The crude fibre, phytic acid and glucosinolate contents were found to be minimum in Bio 21 (6.95%), Bio 2 (3.632%) and Bio 38 (54.324 μmole/g) respectively. Vitamin C content was maximum in Bio 36 and Bio 3. The α-tocopherol content was observed to be maximum Bio 2 (61.875 mg/100g). The total antioxidant activity varied from 5.95 mg AAE/g in Bio 28 to 14.395 mg AAE/g in Bio 39. The DPPH radical scavenging activity was observed to be maximum in Bio 30 and reducing power was found to be maximum in Bio 21(0.867 ± 0.023). The present findings may be utilized for determining the quality status to ascertain their potential for development of better cultivar.

Evaluation for antioxidative and antinutritional characters of yellow- seeded Brassica genotypes.

The minimum contents of antinutritional factors such as crude fiber, phytic acid and glucosinolates were observed in Bio 21, Bio 2 and Bio 38 respectively.

Maximum antioxidant level observed in Bio 2, Bio 28, Bio 30 and Bio 21.

Present finding will be useful for understanding quality status for the development of better cultivars.

Keywords

Yellow seeded Brassica, meal, antioxidative, antinutritional, quality