1Department of Biotechnology, Junagadh Agricultural University, Junagadh-362 001, Gujarat, India
2Department of Gene cs and Plant Breeding, Junagadh Agricultural University, Junagadh-362 001, Gujarat, India
*Corresponding author: harsukhgajera@yahoo.com
Online published on 20 November, 2015.
The present study was carried out for the comparison of RAPD and ISSR markers for polymorphism pa ern and molecular diversity analysis among 17 ridge gourd (Luffa acutangula L.) and sponge gourd (Luffa cylindrica L.) genotypes using 20 RAPD and 12 ISSR markers. Twenty RAPD primers generated total of 94 bands of which 81 bands were polymorphic showing 86.17% polymorphism. The average bands per primer were found 4.05. The polymorphic information content (PIC) was recorded from 0.4828 to 0.8842 for RAPD. Jaccard's similarity coefficient ranged from 30.8% to 78.6% for RAPD. However, out of 30 ISSR primers screened, twelve ISSR primers produced 79 bands of which 66 bands were polymorphic and 83.54% polymorphism with an average of 7.16 bands per primer. The PIC ranged between 0.6548 and 0.8939 for ISSR. Jaccard's similarity coefficient ranged from 22.7% to 81.2% for ISSR. This study showed that RAPD and ISSR markers produced specific DNA fragments for identification of ridge gourd and sponge gourd genotypes.
RAPD and ISSR evident genetic diversity in the range of 30.8 to 78.6% and 22.7 to 81.2%, respectively among ridge gourd and sponge gourd genotypes.
Ridge gourd, sponge gourd, genetic diversity, RAPD, ISSR
