1Department of Agricultural Biotechnology, Anand Agricultural University, Anand - 388 110, Gujarat, India
2Directorate of Medicinal and Aromatc Plants Research, Boriavi, Anand-387310, Gujarat, India
*Corresponding author: push.deore@gmail.com
Online published on 20 November, 2015.
In this study, genetic diversity of 38 accessions of Aloe barbadensis, collected from different parts of India were evaluated using morphological and random amplified polymorphic DNA (RAPD) data. Statistical analysis showed significant differences for all morphological characteristics among the accessions, suggesting that selection for relevant characteristics could be possible. The analysis of molecular diversity was used the RAPD technique, with 14 random primers of 10-mer oligonucleotides. RAPD analysis was performed with 14 primers chosen a er a previous screening. Significant genetic variability among those 38 cultivars was obtained both at the morphological and molecular level. This study demonstrated that for determining the genetic variability among some cultivars, the molecular markers technique is more precise than the morphological traits.
Morphological markers showed the maximum inter cluster distance D = 1.62
14 RAPD produced 89 DNA fragments, with average percentage polymorphism of 82.76.
RAPD and morphological markers were sufficient to assess variability among 38 Aloe genotypes
Aloe, RAPD analysis, dendrogram, genetic diversity
