International Journal of Agriculture, Environment and Biotechnology
  • Year: 2015
  • Volume: 8
  • Issue: 3

Leaf proteome alterations in tolerant pearl millet (Pennisetum glaucum L.) genotype under water stress

  • Author:
  • Tushar J. Anatala, H. P. Gajera, M. K. Mandavia, Rajesh A. Dave, Vishal V. Kothari, B.A. Golakiya
  • Total Page Count: 11
  • Page Number: 539 to 549

Department of Biotechnology, College of Agriculture, Junagadh Agricultural University, Junagadh-362001, Gujarat, India

*Corresponding author: harsukhgajera@yahoo.com

Online published on 20 November, 2015.

Abstract

Drought tolerant pearl millet genotype was used for differential physiological and proteomic analysis. The water stress was imposed on 20 days seedling up to five days. The physiological parameters viz. soil moisture content, relative water content (RWC), shoot length (cm) were studied from drought and control seedling a er 25 days. The results showed significant changes on RWC and soil moisture content was decreased under water stress. Proteome analysis of 2D gel electrophoresis indicates around 1262 well resolved spots within the 4–7 pH and 10–110 kDa ranges. Image analysis revealed the presence of both, qualitative and quantitative changes between two treatments. The proteomic changes were observed in tolerance genotype J-2340 resulted total 84 spots protein (22.5–97.4 kDa, pH- 4.00 to 6.73) matches with control and water stress treatments. However, 32 proteins up regulated (29.0–97.4 kDa, pH 4.20–7.00) and eight down regulated (57.9–97.4kDa, pH 4.00 to 6.68) were observed a er imposing water stress.

The leaf proteome analysis in tolerant pearl millet J-2340 evident 1262 protein spots of which 32 up regulated and 8 down regulated proteins under water stress.

Keywords

Pearl millet, Relative water content, Water stress, 2D gel electrophoresis