International Journal of Agriculture, Environment and Biotechnology
  • Year: 2015
  • Volume: 8
  • Issue: 3

Clonal variability studies in ‘langra’ mango (Mangifera indica L.) using morphological, biochemical and molecular markers

  • Author:
  • Ankita Anu1, Bishun Deo Prasad2,, Ravindra Kumar1, Pankaj Kumar2, Vishwa Bandhu Patel1, Rudra Narayan Jha3
  • Total Page Count: 15
  • Page Number: 567 to 581

1Department of Horticulture, Bihar Agricultural College, Bihar Agricultural University, Sabour, Bhagalpur, Bihar, India

2Department of Plant Molecular Biology and Genetic Engineering, Bihar Agricultural College, Bihar Agricultural University, Sabour, Bhagalpur, Bihar, India

3Department of Statistics, Mathematics and Computer Application, Bihar Agricultural College, Bihar Agricultural University, Sabour, Bhagalpur, Bihar, India

*Corresponding author: dev.bishnu@gmail.com

BAU-communica on no 025–2015

Abstract

Mango is one of the most economically important tropical fruits grown in tropical and sub-tropical regions of the world. Being a deliciousand widelycultivated fruit, it is regarded as ‘the King of fruits'on the Indian subcontinent and the ‘national fruit of India’ because of the flavour and nutritional qualities. Mango has rich intra-specific diversity, with about 1600 and 1000 cultivars of mango present globally and India, respectively. ‘Langra’ is one of the leading commercial mango varieties of Northern India including Bihar which is known for mildly fibrous flesh and with a distinct pleasant taste and turpentine flavour. Significant variation exists among the clones of ‘Langra’ mango with respect to fruit shape, size, colour, quality and taste. Several studies have been made on characterization of intra-varietal variability of many different cultivars of mango. However, clonal variability studies in ‘Langra’ mango are limited. In this present investigation, an a empt were undertaken to study the clonal variability exit in some ‘Langra’ mango using morphological, biochemical and Molecular Markers. These results provide evidence that a significant level of genetic variation exits among 10 clones of ‘Langra’ mango which can be used for mass multiplication of superior clone(s) and can be further utilized in breeding programs.

Ten genotypes of ‘Langra’ mango were characterized and evaluated for variation in different qualitative and quantitative traits.

RAPD analysis was performed to discriminate the different clones of ‘Langra’ mango.

Based on all the analysis Dudhiya Maldah was found to be the best clone of ‘Langra’ mango among all the clones analysed.

Keywords

Ascorbic acid, langra, mangifera indica L, NTSYS, RAPD, total soluble solids (TSS)