1All India Coordinated Research Project on Rapeseed and Mustard (ICAR), Rajmata Vijayaraje Scindia Krishi Vishwavidyalaya, Zonal Agricultural Research Station, Morena, M.P., 476001, India
2Plant Genetic Manipulation Group, Department of Life Science, University of Nottingham, University Park, Nottingham, NG7 2RD, England
*Correspondence author: vkt786@rediffmail.com
Online published on 29 January, 2016.
The high yielding and reproducible micro-method of barley total DNA isolation was developed. A strong buffer was used for the maximum yield of DNA. RNA was removed by treating DNA with DNase-free pancreatic RNase A and polysaccharide contamination was removed by ethanol precipitation. DNA isolated from callus or leaf tissue was proved enough for restriction enzyme digestion and Southern blotting.
Protocol is most suitable for the isolation of DNA from small quantity of tissues
Strong buffer yielded high quantity of DNA.
Barley, callus, DNA, isolation, leaf tissues, tissue culture
