1Faculty of Agriculture, Sher-e-Kashmir University of Agricultural Science and Technology, Chatha-190008, Jammu, India
3Division of Genetics and Plant Breeding, FoA, Sher-e-Kashmir University of Agricultural Science and Technology of Kashmir, Wadura, Sopore-193201, Jammu and Kashmir, India
*Corresponding author: javeedlone09@mail.com
Online published on 4 November, 2016.
The present study entitled “In vitro Regeneration Studies in Brassica napus, variety GSL-1 and DGS-1 was carried out in the Biotechnology Laboratory, Division of Genetics and Plant Breeding SKUAST-J. Brassica napus, variety GSL 1 and DGS-1 were cultured on MS Media supplemented with different concentrations of growth regulators. The sterilization treatment with HgCl2 (0.1%) for 3 minutes resulted in highest per cent of aseptic seeds and survival of seedlings. Highest callus induction was observed in GSL-1. MS medium augmented with 2, 4-D at 2–2.5 mg/l. Different concentrations of growth regulators in media showed a significant difference in the callus induction frequency (CIF) and regeneration frequency (RF) for both the genotypes tested. MS medium supplemented with BAP (5.0 mg/l) and 2, 4-D (0.5 mg/l) showed highest shoot regeneration frequency in GSL-1.
Seed Sterilisation with HgCl2 (0.1%) for three minutes resulted (100%) aseptic callus.
Highest callus induction frequency was observed in GSL-1.
MS medium supplemented with BAP (5.0 mg/l) and 2, 4-D (0.5 mg/l) showed highest shoot regeneration frequency in GSL-1.
In vitro, Brassica, callus induction frequency, regeneration frequency
