Four rumen fistulated animals, 2 each of cattle and buflalo males were fed on weighed quantity of 4% urea treated wheaa bhusa (UTWB) supplemented with known amounts of either groundnut cake (GNC, T1), mustard cake (MC, T2), cotton seed cake (CSC, T3 or linseed cake (LSC, T4) one after another on isonitrogenous basis to meet the maintenance needs. After 21d, samples of rumen fluid were drawn at 0, 2, 4, 6 and 8 h post feeding. The CP disappearance was determined at 0, 6, 9, 12 and d2 4. T1 maintained significantly (P< 0. 01) higher rumen pH (6.58) in comparison to T2 (6.22), T3 (637) and T4 (635). The rumen NH3-N concentration (mg/100 ml SRL) peaked at 2 h post feeding and differed significantly (P< 0.01) between T1 and T3 The total-N (mg/100 ml SRL) and TVFA (mm/l) were different among treatments (P< 0.01). The differences in TCA-ppt ‘N’ (mg/100 ml SRL) were non significant among different treatments. The protozoal and bacterial counts were significant (P< 0.01) among different treatments. The disappearance of CP from the four cakes was higher on T2 followed by on T4 T3 and T1, in decreasing order. The CP disappearance in MC and GNC was much faster than from other cakes and CP disappearance in MC was not affected by dietary treatments. Supplementation of urea treated wheat bhusa diet with GNC or CSC was found to improve rumen fermentation and dtitization of protein.
