Present study was conducted to determine the degradation of vitamin E under in vitro conditions. For this, rumen liquor collected from 3 rumen fistulated buffalo calves was incubated along with substrate at 39°C for 0, 3, 6, 12 and 24 h. Substrates for the in vitro incubations were similar to the diets fed to rumen fistulated buffalo calves. In addition, 1.0 IU α tocopheryl acetate was added to each flask containing 50 ml of the inoculums and 0.5g substrate. Results revealed that tocopherol remained statistically similar in all the in vitro flasks at different hours. It was inferred that there was no degradation of vitamin E under in vitro incubation system using rumen liquor from buffalo calves fed diet with different forage: concentrate ratios.
Vitamin E, In vitro degradation
