Indian Journal of Animal Nutrition

Twelve cultures of Saccharomyces cerevisiae and five cultures ofAspergillus oryzyae were evaluated as probiotic supplement under in vitro condition using rumen liquor from adult buffalo. Each microorganism of twelve cultures individually was added @10⁹cfu/conical flask while keeping one group as control where no supplementation was made. Most of the strains of S. cerevisiae significantly increased the IVDMD.S. cerevisiae 225 increased the IVDMD to 47.16% as compared to 41.99% in control group. However, supplementation ofA. oryzae had no effect on the IVDMD. The TVFA concentration was also higher in S. cerevisiae group and highest value was observed in the group supplemented with strain 189 (7.77 meq/100 ml) followed by 225 (7.63 meq/100 ml). The concentration of ammonia was found lower both inS. cerevisiae and A. oryzae supplemented group except S. cerevisiae 39, 43 andA. oryzae 643 strains. The total gas production was higher in groups supplemented withS. cerevisiae 45, 50,186, 189, 225 and A. oryzae 152 as compared to control group (59.63, 62.37, 61.03, 61.23, 62.80 and 59.77 vs 57.50 ml/g OM digested, respectively) The methane production was less in A. oryzae 1122 supplemented group (14.06ml/g of OM digested), however, other strains of A. oryzae has no impact on the methane production. The methane production was also lower in groups supplemented with S. cerevisiae 41, 43, 45, 46, 50, 186, 189 and 225(14.60, 14.17, 12.60, 13.53, 12.87, 14.23, 14.00 and 13.2 ml/g of OM digested. The numbers of total bacteria was higher in groups supplemented withS. cerevisiae strains 42, 43, 45, 47, 50, 186, 189 and 225 and A. oryzae strains 152, 643, 1122 and 1846.