Effect of Supplementation of Rumen Protected Lysine plus Methionine or Choline on Blood Biochemical Parameters in Crossbred Cows

Eighteen Karan Fries cows with most probable producing ability (MPPA) of around 4233 kg milk production were divided into 3 groups of 6 animals each in order to study the effect of rumen-protected lysine (RPL) plus methionine (RPM) and choline (RPC) on blood biochemical parameters. All the animals were fed basal diet consisting of wheat (Triticum aestivum) straw (20%), chaffed green maize (Zea mays) fodder (40%) and concentrate mixture (40%) on DM basis to meet the requirements (NRC, 2001). The animals in group T₁ (control) were given no supplement while those in groups T₂ and T₃ were given 7 g RPM + 6 g RPL and 60 g RPC per day, respectively. Supplementation of RPM + RPL and RPC resulted in higher (P<0.05) blood glucose and total plasma immunoglobulin concentration and lower (P<0.05) aspartate amino transferase activity compared to control group while there was no significant effect on alanine amino transferase activity due to supplementation, however, the values of all these parameters were within physiological linits.