Rapid Estimation of Chlorpyrifos in Feed Samples using Gas Chromatograph-Micro Electron Capture Detector (GC-µECD)

In this research paper, we have standardized a rapid method for estimation of chlorpyrifos residues in feed samples using Gas Chromatograph-Micro Electron Capture Detector(GC-µECD). The extraction method used for this study was QuEChERS viz. quick, easy, cheap, effective, rugged and safe. Validation was done by performing the following parameters; Linearity, Limit of detection (LOD), Limit of quantification (LOQ), Matrix effect and Recovery percentage. A regression equation with regression coefficient (r2) of 0.9913 was obtained indicating excellent linearity. LOD and LOQ of the method were obtained as 0.1 mg/L and 0.4 mg/L respectively. Recovery percentage of the spiked concentration 0.1 ppm was obtained as 85.7% and for 0.5 ppm it was 77.7%. The method qualified the required parameters for analysis of chloropyriphos in the different classes of feeds. Different classes of feedstuffs were analyzed using this method.