Effect of Urea and Exogenous Fibrolytic Enzyme Treated Paddy Straw on In Vitro Rumen Fermentation Characteristics and Degradability

This study was conducted to evaluate the effect of urea and exogenous fibrolytic enzyme (EFE) treatment on in-vitro digestibility and fermentation attributes of paddy straw. In this study, in vitro degradability of nutrients in paddy straw as an effect of treatment of urea and EFE at various levels (1.0, 2.0, 3.0, 3.5, 4.0, 4.5, 5.0, 6.0, 7.0, 8.0 and 9.0 % of urea and 1.0, 2.0, 3.0, 4.0, 5.0, 6.0, 7.0, 8.0 and 9.0 g/kg DM of EFE) were tested. For selecting the best levels of urea and EFE, test samples were incubated with strained rumen liquor and different dosages levels were studied on rumen fermentation parameters like total gas production along with in vitro DM and OM digestibility, ammonia nitrogen, pH and some calculated parameters such as partitioning factor (PF), microbial biomass production (MBP) and efficiency of microbial protein synthesis (EMPS) by incorporation of urea and EFE treated paddy straw. The result showed significant improvement on in vitro dry matter digestibility (IVDMD), in vitro organic matter digestibility (IVOMD), in vitro total gas production (IVTGP), MBP and PF at 4% and 8 g/kg DM of urea and EFE treatment level in comparison to other levels. The optimum results were obtained at 4% and 8 g/kg DM of urea and EFE treated paddy straw, hence a 4% and 8 g/kg DM dosage, respectively may be selected for in vivo study.