Studies were conducted to examine the effect of substitution of serum with cystic follicular fluid in the IVM media and also to test the efficacy of cystic follicular fluid (buCFF) at 50 and 100 per cent levels for in vitro maturation of buffalo oocytes. Results indicated that buffalo cystic follicular fluid supplemented with PMSG possess the ability for induction of maturation of buffalo oocytes and could be successfully tried as buffalo oocyte maturation medium. Buffalo cystic follicular fluid as a whole maturation medium also has the ability to induce the cumulus expansion which may be cost effective for the IVM of buffalo oocytes.
