Department of Animal Reproduction, Gynaecology and Obstetrics, College of Veterinary and Animal Sciences, Pantnagar - 263 145, India.
*Part of M.V.Sc. thesis submitted by first author to G.B. Pant University of Agriculture and Technology, Pantnagar.
An experiment was conducted to study the freezability of unwashed and washed buffalo spermatozoa in egg yolk tris (EYT) dilutor modified by addition of 0, 3, 6 and 10% fetal calf serum (EYT-So, EYT-S1, EYT-S2 and EYT-S3, respectively). Acrosome reaction test, in addition to sperm motility, livability and total sperm abnormalities, was performed at 0 hr (after dilution), PF (pre-freezing/after equilibration) and PT (post thaw) stages to evaluate the dilutors for maximum recovery of post-thaw fertilizing ability, motility and livability. Washing significantly (P < 0.01) affected acrosome reaction percentage, sperm motility, livability and total abnormalities. Dilutors were non-significant on all parameters, whereas acrosome reacting spermatozoa were higher in EYT-S3 dilutor for unwahed and in EYT-S2 dilutor for washed samples. PT motility and livability were higher in EYT-S2 dilutor as compared to control for both unwashed and washed samples. Total sperm abnormalities were within normallirnits in all dilutors at all stages.
