Serum transminases and phosphatases were assayed in fertile and infertile cattle and buffalo bulls. Serum glutamate pyruvate transaminase (SGPT) did not differ significantly in relation to fertility and species. Serum glutamate oxaloacetate transaminase (SGOT) was higher in infertile than fertile bulls in both the species. The values were higher in cattle than buffalo bulls. The differences were non-significant. Acid phosphatase did not differ significantly in relation to fertility though the value was 20.28 per cent higher in infertile buffalo bulls. Higher values were obtained in buffalo (3.85±0.68 KA units) compared to cattle 1.91±0.51 KA units). Alkaline phosphatase was higher by 90.99 per cent in cattle and 44.67 per cent in buffalo infertile bulls. The values were significantly (P<0.01) higher in buffalo bulls (9.53±0.93 KA units) compared to that in cattle (4.84±0.74 KA units). It is concluded that serum acid- and alkaline phosphatase activities were elevated in infertile bulls and relatively more in buffalo compared to cattle bulls. The role of this enzyme in male reproduction and in different species of livestock needs further elucidation.