1Department of Gynaecology, Obstetrics and A.I., College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati-781 022, India.
2Nartap State Veterinary Dispensary, Sonapur, Kamrup, Assam.
3Department of Anatomy and Histology, C.V.Sc., AAU, Khanapara.
A total of 36 ejaculates collected from four adult crossbred (Beetal x Assam Local) goats were used to record the Lactic Dehydrogenase(LDH) reactivity in mid piece of spermatozoa during different stages of cryopreservation. Seminal ejaculates were divided equally and extended in Tris-egg yolk-citric acid-glycerol extender containing either fructose, sucrose or lactose and frozen in liquid nitrogen using French medium straw after allowing glycerol equilibration period of either 11/2, 1 or 1/2 hour before freezing. The study revealed that the LDH reactivity of spermatozoa was comparable when frozen after different glycerol equilibration periods suggesting that out of the three, any convenient glycerol equilibration period could be adopted without affecting the quality of frozen semen. The reactivity of LDH in spematozoa was higher when frozen using Tris containing fructose which might be indicative of better cryopreservation provided by the extender.
