Enzymatic profiles of alkaline and acid phosphatases in the buffalo ovarian antral follicular fluid were studied using ovaries procured from Civil Slaughterhouse, Bangalore. Antral follicular fluid aspirated from small (<6 mm), medium (6–10 mm) and large (11–16 mm) follicles was analyzed for phosphatase activity using clinical analyzer (photometer, BT-224 Biotechnica instruments). The acid phosphatase activity decreased with advancement in follicular size reaching the lowest value in large follicle group. Similar trend was observed with respect to the alkaline phosphatase activity. The acid and alkaline phosphatase activities were highest during the hotter months of April and June when the monthly average mean temperatures were (27.20°C) and (25.85°C) respectively. It was lowest during the cooler months of December and January, when the ambient temperatures were (21.20°C) and (20.95°C) respectively. However during July and August, the activity was intermediate. From the study it was concluded that the enzyme activity decreased with the follicular development and it increased with the increase in ambient temperature.
