Animal Genetics and Breeding Division, National Dairy Research Institute, Karnal-132001, India.
Cryopreserved spermatozoa are prone to cryoinjury which induces membrane damage and render them incapable to undergo acrosome reaction (AR). In the present study, cryopreserved semen samples obtained from 30 Karan Fries bulls were subjected to in vitro AR. The AR scores were correlated with the fertility rates (conception rates) following A.I. with frozen semen frozen semen. The AR scores and gross seminal parameters showed significant correlations except for semen volume. A prediction equation (R2 = 37.3) was constructed from AR scores and was used to predict the conception rates of donor bulls. The predicted and observed conception rates did not show any significant difference for chi square test. The study indicates that in vitro acrosome reaction test may be complemented with the conventional semen analyses tests prior to using cryopreserved semen samples for AI purpose.
