College of Agricultural Biotechnology, Latur, (M.S.), India.
1Part of Ph.D. thesis submitted by Senior Author.
Isolation of pure high molecular weight genomic DNA is a pre-requisite in microsatellite studies. The phenol-chloroform method of DNA extraction from whole blood usually involves several steps which are time consuming. Further the phenol is corrosive and toxic. A simple procedure called High salt method was further optimized for isolation of high molecular weight genomic DNA from much lesser quantity of buffalo blood i.e. only from 200 μl, which helped in avoiding wastage of blood sample and also saving costly chemicals. The optimized High salt method produced good yield of pure high molecular weight genomic DNA.
