For the present study bulbourethral glands were obtained from 28 castrated and uncastrated cattle of Deoni breed and other non-descript breeds. The glycogen activity was intensely positive, acid muco-polysaccharides and amyloid reaction were intense to moderate, the epithelial lining of the alveoli showed mild to moderate alkaline phosphates activity in uncastrated cattle. However, the glycogen activity was mild to moderate, acid muco-polysaccharides showed moderate activity, the mild activity for amyloids was observed in castrated cattle. There was no reaction for alkaline phosphates activity in the castrated cattle.