In order to determine the optimum concentration of caffeine and incubation period for in vitro capacitation of frozen bovine spermatozoa, washed spermatozoa were incubated in sperm-TALP (Tyrode's albumin, lactate and pyruvate) having bovine serum albumin @ 6mg/ml along with supplementation of caffeine @ 0, 5, 10 and 20 mM for 0, 30, 60 and 90 min. In vitro capacitation was evaluated on the basis of hyperactive motility, head to head agglutination and acrosome reaction of spermatozoa. The highest rate of capacitation was observed with 20 mM caffeine at 90 min. of incubation.
Caffeine, Bovine, Incubation period, Spermatozoa
