Indian Journal of Animal Research
SCOPUSWeb of Science
  • Year: 2013
  • Volume: 47
  • Issue: 2

Genetic variability of BoLA-DRB3.2 gene in Kenkatha breed of cattle assessed by PCR-SSCP

  • Author:
  • Rakesh Ranjan, C.D. Bhong1, K.N. Chavan2, S.N.S. Parmar, C.G. Joshi3
  • Total Page Count: 4
  • Page Number: 164 to 167

1KNP College of Veterinary Science, Shriwal, Maharastra.

2SDAU, S.K. Nagar, Gujrat.

3MU, Anand

Animal Biotechnology Centre, MPPCVV, Jabalpur, 482 004, India.

*Corresponding author's e-mail: rkrbiotech@gmail.com

Online published on 13 May, 2013.

Abstract

Present study was conducted to investigate BoLA (Bovine leucocyte antigen) gene polymorphism in indigenous draft Kenkatha (n=52) breed of cattle at DRB3.2 loci by PCR-SSCP (Single strand conformational polymorphism). This BoLA region encodes cell surface glycoprotein, which binds to foreign antigens of T lymphocytes that play central role in the induction and regulation of adaptive immunity. A PCR-SSCP of 284bp amplicon of BoLA-DRB3.2 gene revealed 13 SSCP patterns. This suggests that exon 2 region of BoLA-DRB3 is highly polymorphic in nature. This may be due to natural selection on a BoLA allele pool, leading to the development of resistance to local infectious diseases.

Keywords

BoLA, Kenkatha, PCR-SSCP