Indian Journal of Animal Research
SCOPUSWeb of Science
  • Year: 2013
  • Volume: 47
  • Issue: 3

Determination of alleles of the ovine PrNP gene using PCR-single strand conformational polymorphism analysis in Malpura sheep

  • Author:
  • Sharda Choudhary1,, Neelam Gupta2
  • Total Page Count: 3
  • Page Number: 265 to 267

1NRC on Seed Spices, Ajmer-305 206

2ICAR, New Delhi-110 001

National Bureau of Animal Genetic Resources, Karnal-132 001, India

*Corresponding author's e-mail: shardaajmer@yahoo.com; neelamgnbagr@gmail.com

Abstract

SSCP polymorphism was detected of ovine PRNP coding region exon-3 in 52 blood samples from Malpura sheep breed (Ovis aries) of semi-arid region of India. Three PCR-SSCP amplicons with different nucleotide base pairs were screened. In primer set I covering 19–373 nucleotide showed 6 types of haplotype, primer set 2 covering nucleotide region from 354–711 showed 9 types of haplotype (A-I) and primer set 3 nucleotide region from 692–1001 showed 9 types of haplotypes. The data will help for the detection of SNP markers related to disease resistance or susceptibility in India.

Keywords

Allelic variants, Prion protein gene, Sheep, Single-strand conformation polymorphism