Screening of dairy breeding bulls for chromosomal profile and its andrological attributes

Reproductive efficiency is a critical component and the most crucial issue faced by the dairy industries in India and abroad. The present study aimed at assessing the occurrences of male spermatological anomalies leading to poor reproductive performance and to study the chromosomal profile (G-banding) in dairy breeding bulls. Hypo-osmotic swelling test (HOST) and in vitro acrosome reaction were studied using fresh ejaculates of dairy breeding bulls. The metaphase plates did not reveal any chromosomal anomaly (either structural or numerical) in both the species. HOST results indicated significant differences between categories (high and low post thaw motility) but the scores did not differ significantly between the species. In vitro acrosome reaction showed that the bovine species showed significant difference (p<0.05); between various incubation periods (viz. 0, 4 and 6 hours) and the categories differed significantly between nonreacted grades spermatozoa (p<0.01). Although, the present study could not identify any chromosomal anomaly, in generally, the breeding bulls maintained for semen production, should be screened for cytogenetic profile and the sperm membrane integrity.