1Central Avian Research Institute, Izatnagar, Bareilly, Uttar Pradesh-243 122, India
2Madhavaram Milk Colony, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu-600 051, India
3Department of Poultry Science, Livestock Research Complex, Tamil Nadu Veterinary and Animal Sciences University, Orthanadu, Thanjavur
4Department of Pharmacology, Veterinary University Training and Research Centre, Tamil Nadu Veterinary and Animal Sciences University, Thanjavur
Department of Poultry Science, Madras Veterinary College, Chennai-600 007, Tamil Nadu, India
*Corresponding author's e-mail: darshanabhaisare@gmail.com
Online published on 19 August, 2016.
Two in-vitro experiments were conducted to evaluate the antibacterial, antifungal and antiviral properties of essential oil of herbal seeds. In-vitro antimicrobial properties of essential oils of phytobiotics was determined by disc diffusion method against Escherichia coli, Staphylococcus aureus, Pasteurella multocida and Salmonella typhi and two fungi namely Aspergillusfumigatus and Candida albicans. Thyme oil had statistically similar or significantly (P<0.05) higher inhibition zone against all the bacterial and fungal species compared to standard antibiotic, chloramphenicol or antifungal drug, nystatin. Fenugreek oil was not found to be active against microbes compared to control. Fennel and cumin oils had statistically similar or significantly (P<0.05) higher inhibition zone against all microbes except Staphylococcus aureus and Salmonella typhi compared to control. Antiviral activity of essential oils was determined by anti-NDV assay against New Castle disease virus (LaSota). The results indicated that essential oils of herbal seeds do not possess antiviral activity against NDV LaSota virus at 1mg/ml concentration.
Cumin, Essential oils, Fennel, Fenugreek, In-vitro antimicrobial, Thyme
