Influence of FASN gene polymorphism on milk production and its composition traits in Murrah buffaloes

In present study the effect of genetic polymorphism of Fatty acid synthase (FASN) gene on eight traits related to milk production and its composition in 162 Murrah buffaloes was studied. The traits studied includes 305 days milk yield, lactation fat average (LFA), 305 days fat yield (305LFY), lactation solid not fat average (LSA), 305 days solid not fat yield (305LSY), lactation total solid average (LTSA), 305 days total solid yield (305LTSY) and peak yield (PY). Restriction Fragment Length Polymorphism (RFLP) was used to identify the SNP in a 472 bp PCR amplified product of exon-40 in FASN gene. It was found to be polymorphic with Guanine to Adenine transition and three genotypes namely AA, AG and GG were observed. Allele A was found to be more frequent than G allele. We report for the first time that exon-40 FASN gene is associated with LFA, LTSA and peak yield in Murrah buffaloes. This information can augment future studies to determine the role of bovine FASN gene as a candidate gene marker for a milk fat, total solids and peak milk production.