Indian Journal of Animal Research

In order to investigate action mechanism of Skp2 in SD rat stress model continuously challenged with LPS, not only the histopathological changes but also the distribution and expression of Skp2 and P27^Kip1 were detected in lungs from SD rats by HE and immunohisto chemistry staining respectively. According to present results, Skp2 and P27^Kip1 mianly distributed in the epithelia of respiratory bronchioles, pseudostratified columnar ciliated epithelia of bronchus and vascular endothelial, a few were located at alveolar wall. As well, area, IOD and IOD/area of Skp2 observably increased in LPS-treated rats (P<0.05 or P<0.01), but IOD and IOD/area of P27^Kip1 apparently decreased in LPS-treated rats (P<0.05 or P<0.01). In conclusion, moderate pathological changes existed in lungs from SD rat stress model challenged by LPS. Moreover, Skp2 high level was negatively correlated with P27^Kip1 low expression in lungs, it is indicated that Skp2 probably became a key indicator during stress disease process.