1Faculty of Veterinary Medicine, Balikesir University, Balikesir, 10100, Turkey
Balikesir University, Faculty of Veterinary Medicine, Department of Anatomy, Balikesir, 10100, Turkey
2Faculty of Veterinary Medicine, Mustafa Kemal University, Hatay, 3100, Turkey
3Faculty of Medicine, Karabük University, Karabük-181004, Turkey
4Faculty of Medicine, Abant Izzet Baysal University, Bolu,14000, Turkey
This study documented macroscopic and microscopic features and mineralization of the caudally located tracheal rings and syrinxin twoostrich (struthio camelus) having three years of age. The syrinx andtrachea of the birds werestained in toto with alcian blue and alizarin red for cartilage and mineralization. Observations on the syrinx and trachea, measurements and photography were performed under stereo-microscopy. They were stained grossly using alizarin red and alcian blue to visualize mineralization and histologically by hemotoxylin and eosin (H&E) to detect ossification areas, if any. Results revealed incomplete tracheal rings located caudally in-between the intact ones. Alizarin red and alcian blue staining displayed mineralized regions grossly. Histological slides by H&E staining showed no ossification. Overall results proposed that alizarin red and alcian blue double staining is a good toll to determine mineralization which is calcium accumulation in the tissue before the formation of bone cells.
Mineralization, Ostrich, Syrinx, Trachea
