Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science, P.V. Narsimha Rao Telangana Veterinary University, Rajendranagar, Hyderabad-500 030, Telangana, India
*Corresponding author's e-mail: rsri0835@gmail.com
Online published on 2 January, 2019.
To investigate the preservability of Deccani ram semen in three different extenders-SC (Sodium Citrate), T (Tris), C (Coconut milk) egg yolk extenders after dilution and chilling (5ºC) at 1, 24 and 48 hr. of storage. Eight Deccani adult rams (aged 2–4 years) were selected and ejaculate from each ram was divided into 3 aliquots and diluted with SC, T and C extenders (final concentration-400 million spermatozoa/0.2 ml semen) and stored at 5ºC. Semen evaluation was done by computer assisted semen analyser (CASA). Analysis of Variance (ANOVA) was used to test the significance difference between extenders. S: Sodium citrate (SC) and Tris (T)-Egg yolk extender was significantly (P<0.05) better as compared to Coconut milk extender (C). No significant difference (P>0.05) was observed between SC and T. Sperm kinematics assessment by CASA revealed Sodium citrate-based egg-yolk extender (SC) as better extender for preservation of Deccani Ram semen upto 48 hr. of storage as compared to T and C.
Computer assisted semen analysis, Liquid preservation, Ram semen, Sperm kinematics
