1AGB Division, ICAR-NDRI, Karnal-132 001, Haryana, India
Department of Veterinary Physiology and Biochemistry, College of Veterinary Science and Animal Husbandry Narendra Deva University of Agriculture and Technology, Kumarganj, Faizabad-224 229, Uttar Pradesh, India
*Corresponding author's e-mail: dr.shivkumarjnp@gmail.com
Online published on 13 March, 2018.
Present study was carried out to study egg production performance and polymorphism of Prolactin gene at 24 bp indel locusat promoter region (PRL24). Egg production performances were recorded asage atfirst egg(AFE), BodyWeight at First Egg (WFE), Mean Egg Weight (MEW) and Total No. of Eggs at 90 days of laying (TEN). DNA was isolated from blood of 20 Kadaknath birds collected from wing vein. PRL24 locus for indel polymorphism was amplified by PCR and the product was resolved on 6% native PAGE for genotyping. The AFE (d), WFE (Kg), MEW (g) and TEN of Kadaknath hens in the present study were found to be 188.00±0.71, 1.26±0.03, 42.83±0.21 and 37.75±0.59 respectively. The Prolactin gene locus PRL24 showed two alleles I and D and three genotypes: II, ID and DD. The frequencies of I and D alleles at this locus were 0.55 & 0.45 respectively. The birds of D allele had a significantly (P<0.05) better TEN than birds of I allele.
Egg production performance, Kadaknath, Prolactin, Polymorphism
