Indian Journal of Animal Research

SCOPUSWeb of Science
  • Year: 2018
  • Volume: 52
  • Issue: 3

Phylogenetic analysis based studies on genetic variation of Cytochrome b gene of Indian peafowl (Pavo cristatus) in Pakistan

  • Author:
  • Junaid Naseer1,, Khalid Mahmood Anjum1, Waseem Ahmad Khan1, Muhammad Imran2, Muhammad Ishaque3, Shahid Hafeez3, Muhammad Asif Munir1, Muhammad Awais Nazir1
  • Total Page Count: 4
  • Page Number: 343 to 346

1Department of Wildlife and Ecology, University of Veterinary and Animal Sciences, Lahore-Pakistan

Department of Wildlife and Ecology, University of Veterinary and Animal Sciences, Lahore-Pakistan

2Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore-Pakistan

3Department of Forestry and Range Management, University of Agriculture, Faisalabad-Pakistan

Abstract

This study was designed to analyze genetic variation between Indian peafowl available at different locations by targeting the Cytochrome b gene. A total of ten birds (n=10) were selected randomly for sample collection. Five birds were selected from government sector and five from private breeding farms. DNA was extracted, purified and measured by using Nano drop. Extracted DNA was amplified using universal primers targeting Cytochrome b gene on polymerase chain reaction (PCR). PCR product was run on a gel for the desired DNA bands. DNA from gel was eluted and sent for sequencing. The sequences were compared with a reference reported sequence of cyt b gene of Indian peafowl with Accession No. L08379.1to find out the genetic diversity. Indian peafowl of government sector showed more similarity ≥95% rather than bird of private sector with ≥90% homology with reference Accession No. More genetic variation, which isthe guarantee of resistance to disease and environmental fitness among the Indian peafowl at private sector, might be due to random reproductive behavior.

Keywords

Cytochrome b gene, Genetic variation, Indian Peafowl